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. 2018 Sep 27;10(10):525. doi: 10.3390/v10100525

Figure 2.

Figure 2

The effects of BoHV-1 infection on the expression of HATs (histone acetyltransferase) and HDACs (histone deacetylases). (A) MDBK cells in 60 mm dishes were mock infected or infected with BoHV-1 at an MOI of 1 for 4, 12 and 24 h. The cell lysates were then prepared for Western blots to detect (CREB binding protein and p300), GCN5L2 (general control of amino acid synthesis yeast homolog like 2) and PCAF (P300/CBP-associated factor). Data shown are representative of three independent experiments. (B,D) The relative band intensity was analyzed with software image J, and each analysis was compared with that of uninfected control which was arbitrarily set as 100%. The error bars denote the variability between the three independent experiments. (C) MDBK cells in 60 mm dishes were infected with BoHV-1 at an MOI of 1 for 4, 12 and 24 h. The cell lysates were then prepared for Western blots to detect HDAC1, HDAC2, HDAC3 and HDAC4. Data shown are representative of three independent experiments.