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. 2018 Oct 15;10(10):1507. doi: 10.3390/nu10101507

Figure 5.

Figure 5

Glucose and fatty acid accumulation by BeWo cells after 1-h incubation. (a) The luminescence intensity of labeled glucose that was accumulated in the BeWo cells; (b) The fluorescence intensity of labeled fatty acids that were accumulated in the BeWo cells. BeWo cells were treated with 1 mM choline (CS), 1mM betaine (BS), or saline control and with 30 mM glucose (HG) or 30 mM mannose (NG) in the Minimum Essential Medium for 48 h, followed by 1-h incubation with the labeled glucose or fatty acid probes. Each experiment was done in triplicate and repeated 3 times. Values are mean ± standard error of mean (SEM); a, b, c: any two groups with no overlapping characters have a statistically significant difference (p < 0.05) between them. Solid bars: NG-CO; shaded bars: HG-CO; open bars: HG-CS; hatched bars: HG-BS. NG: normal glucose; HG: high glucose; BS: betaine supplemented; CO: untreated control; CS: choline supplemented.