Figure 3.

Cytokine profiles of splenic CD4⁺ T cells recovered from mice vaccinated with SjLD1, SjTPI and SjLD1 + SjTPI adjuvanted with QuilA at 6 weeks post-challenge. Splenocytes isolated from vaccinated and adjuvant control mice were stimulated with media, rSjLD1, rSjTPI, SEA and SWAP for 72 h, followed by intracellular cytokine staining. The proportion of IFNγ- and IL-4-producing splenic CD4⁺ T cells were determined after stimulation with (a) rSjLD1; (b) rSjTPI; (c) SWAP and (d) SEA (* p value ≤ 0.05; ** p value ≤ 0.001).