Table 1.
Parasitologic data for vaccinated and control mice challenged with 34 ± 1 Schistosoma japonicum cercariae.
| Adjuvant | Group | Number Adult Worms Mean ± SE | Mean Length of Adult Worms (mm) Mean ± SE %Reduction (p Value) | Liver Eggs/g Mean ± SE %Reduction (p Value) | Intestinal Eggs/g Mean ± SE %Reduction (p Value) | Maturity of Intestinal Eggs in Stage V (%) Mean ± SE %Reduction (p Value) | Faecal Eggs/g/f Mean ± SE %Reduction (p Value) |
|---|---|---|---|---|---|---|---|
| QuilA. | Control n = 10 | (F) 5.9 ± 0.7 (M) 8.5 ± 0.8 |
(F) 10.4 ± 0.2 (M) 6.6 ± 0.2 |
41886 ± 6001 | 64483 ± 9998 | 21.6 ± 2 | 1398 ± 474 |
| SjLD1 n = 10 |
(F) 3.3 ± 0.6 44% ** (p = 0.008) (M) 4.8 ± 0.8 43.5% * (p = 0.03) |
(F) 10.8 ± 0.3 (M) 7.7 ± 0.3 |
23285 ± 4834 44% * (p = 0.03) |
34533 ± 8038 46% * (p = 0.04) |
8.9 ± 1.5 58% * (p = 0.04) |
546 ± 276 61% * (p = 0.02) |
|
| SjTPI n = 10 |
(F) 5.7 ± 0.6 (M) 7.4 ± 1.0 |
(F) 10.7 ± 0.3 (M) 8.3 ± 0.3 |
42894 ± 4548 | 55473 ± 7574 14% ns (p = 0.4) |
4.4 ± 0.3 79% *** (p = 0.0002) |
776 ± 354 44% ns (p = 0.36) |
|
| SjLD1 + TPI n = 10 |
(F) 5.7 ± 0.9 (M) 8.2 ± 1.1 |
(F) 10.6 ± 0.2 (M) 7.1 ± 0.3 |
41649 ± 4391 | 61291 ± 12505 | 8.4 ± 2.3 67% * (p = 0.03) |
885 ± 320 36% ns (p = 0.32) |
|
| ISA-720 | Control n = 10 |
(F) 5.3 ± 0.9 (M) 11.1 ± 1.6 |
(F) 11.1 ± 0.4 (M) 6.9 ± 0.1 |
36625 ± 2271 | 78969 ± 9486 | 26.6 ± 1.8 | 4278 ± 1019 |
| SjLD1 n = 10 |
(F) 3.7 ± 0.7 30% * (p = 0.05) (M) 12.2 ± 1.1 |
(F) 9.5 ± 0.2 13% ** (p = 0.003) (M) 5.8 ± 0.1 16% *** (p < 0.0001) |
16233 ± 3276 56% *** (p = 0.0003) |
41331 ± 10912 48% * (p = 0.013) |
9.9 ± 2.0 63% * (p = 0.01) |
1349 ± 459 68% * (p = 0.05) |
|
| SjTPI n = 10 |
(F) 5.8 ± 0.7 (M) 12.7 ± 1.7 |
(F) 9.8 ± 0.2 10% ** (p = 0.009) (M) 5.7 ± 0.1 18% *** (p < 0.0001) |
28785 ± 3306 21% ns (p = 0.08) |
74104 ± 10278 | 8.6 ± 1.1 68% *** (p = 0.0007) |
2089 ± 410 51% ns (p = 0.06) |
|
| SjLD1 + TPI n = 10 |
(F) 5.8 ± 1.1 (M) 12.8 ± 1.4 |
(F) 8.7 ± 0.2 20% *** (p < 0.0001) (M) 5.4 ± 0.1 22% *** (p < 0.0001) |
24735 ± 3558 33% * (p = 0.03) |
53788 ± 10381 32% * (p = 0.05) |
11.8 ± 0.9 56% * (p = 0.06) |
2158 ± 399 50% ns (p = 0.07) |
F, female worm; M, male worm; n, the number of mice per group that survived the trial and were necropsied; ns, not significant; SE, standard error of the mean; * p value ≤ 0.05; ** p value ≤ 0.001; *** p value ≤ 0.0001.