Figure 1.

Seven days after induction of AOH, there is a diffuse loss of retinal ganglion cells. (A‐A') Retinal photomontages showing Brn3a⁺ RGCs in a control (A, left) and an experimental (A, right) retina and their isodensity maps (A'). Maps in this figure show that in control retinas, RGCs are denser in the medial–central retina, being densest above of the optic nerve. A diffuse loss of RGCs is observed after AOH. At the bottom of each map is shown the number of RGCs quantified in its corresponding retina. Density colour scale is shown at the bottom of panel A' right and goes from 0 RGCs/mm² (purple) to ≥3500 RGCs/mm² (red). (B) Bar graph showing the mean ± standard deviation of RGCs in control and AOH retinas (n = 6/group). The loss of RGCs in the AOH retinas is significant compared to control (t‐test, p‐value <0.0001). AOH, acute ocular hypertension; S, superior; N, nasal; T, temporal; I, inferior.