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. 2016 Aug 18;95(1):e10–e21. doi: 10.1111/aos.13227

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© 2016 The Authors. Acta Ophthalmologica published by John Wiley & Sons Ltd on behalf of Acta Ophthalmologica Scandinavica Foundation.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Increased number of microglial cells in the ganglion cell layer after acute ocular hypertension (AOH). (A) Magnifications from flat‐mounted retinas immunodetected for Brn3a (RGCs) and Iba1 (microglial cells) and focused on the ganglion cell layer. In control retinas, microglial cells are ramified and evenly distributed. In AOH retinas, microglial cells are amoeboid, less ramified and more abundant. (B) Graphs showing the mean density (left) and the calculated total number (right) ±standard deviation of microglial cells in control or AOH retinas (see Methods for details). The number of microglial cells in the AOH retinas is significantly higher than in control retinas (n = 6/group; t‐test, pvalue <0.0003).