Table 8.
The primer sequences for hepatic mRNA measurements via quantitative real-time RT-PCR (qRT-PCR). Forward and reverse primers, as well as their specific annealing temperatures, were used for qRT-PCR measurements of mRNA levels in the total RNA samples extracted from gilthead sea bream liver.
| Primer | Sequence 5′ → 3′ | Annealing Temperature (°C) |
|---|---|---|
| ∆6-D a* forward | GCAGGCGGAGAGCGACGGTCTGTTCC | 65 |
| ∆6-D a* reverse | AGCAGGATGTGACCCAGGTGGAGGCAGAAG | 65 |
| β-actin b* forward | TCCTGCGGAATCCATGAGA | 60 |
| β-actin b* reverse | GACGTCGCACTTCATGATGCT | 60 |
| PPARα c* forward | TCTCTTCAGCCCACCATCCC | 61 |
| PPARα c* reverse | ATCCCAGCGTGTCGTCTCC | 61 |
| ECH d§ forward | GCCCAAGAAGCCAAGCAATCAG | 60 |
| ECH d§ reverse | CTTTAGCCATAGCAGAGACCAGTTTG | 60 |
| CEL e§ forward | GCTGAGGAGATTGCTCTGAAGGT | 62 |
| CEL e§ reverse | CAGGAAGCCATAGTCTCACCAGTG | 62 |