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. 2018 Oct 20;10(10):574. doi: 10.3390/v10100574

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) immunoblot analysis of cell lysates following transduction with the named BacMam vector in HK-2 (Ai,Aii) or EndoC-βH3 (Aiii) cells at 72 hpt using anti-EGFP or anti-BCL2 antibodies. Molecular weight markers are in kDa. (B) A bar graph was constructed using GraphPad Prism to show the relative band intensities for (Bi) EGFP in HK-2, (Bii) BCL2 in HK-2 and (Biii) BCL2 in EndoC-βH3 cells obtained by band densitometry. Each sample was normalised against a β-actin loading control. Error bars represent ± SD (n = 3) and indicate a significant difference between CMV.EGFP^FB and CMV.EGFP^HT, or CMV.BCL2^FB and CMV.BCL2^HT transduced cells using a Student’s t-test (p < 0.05).