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. 2018 Oct 20;10(10):574. doi: 10.3390/v10100574

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) human pancreatic islets isolated from cadaveric donor 1 (Ai) and donor 2 (Aii) were transduced with BacMam viruses at 250 MOI as shown. Bright field (left) and fluorescent (right) images were taken at 48 hpt using a Zeiss Axiovert 135 inverted epifluorescence microscope (×10). Scale bar, 100 nm. (B) Cell lysates were harvested from donor 1 (Bi) and donor 2 (Bii) at 24 and 48 hpt and were analysed by Western blotting using EGFP-specific antibodies. Molecular weight markers are in kDa.