Figure 3.

Atherosclerosis lesion size was decreased by 10,12 CLA, but not CR. Mice were fed either chow or HFHS diet for 12 weeks, then continued on the indicated diets for an additional 8 weeks. (A,B) Aortas were prepared en face (A), stained with Sudan IV, and lesions imaged and quantified using Image Pro Plus software (B). (C–G) Sections through the aortic sinus of the heart were stained with Movat’s pentachrome, and lesion area (C), collagen (D), and necrotic core area (F–G) was quantified using Image Pro Plus software. Data are presented as mean ± SEM, n = 5–10 mice/group. * p < 0.05 from HFHS control.