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. 2018 Oct 5;19(10):3039. doi: 10.3390/ijms19103039

Figure 3.

Figure 3

Detection of T1 transgenic plants by PCR. PCR analysis of genomic DNA of T1 transgenic plants using bar gene primers. The length of PCR product is 427 bp. M: DL2000 Plus; CK+: plasmid DNA; WT: wild-type soybean plant; lanes 1–7: T1 lines (line 1-1, line 1-2, line 2-1, line 3-1, line 3-3, line 3-4, line 4-1) versus 1–4 T0 lines from treatment without l-amino acid;lanes 8–15: T1 lines (line 6-1, line 6-2, line 7-1, line 7-2, line 7-3, line 7-4, line 7-5, line 8-1) versus 6–8 T0 lines from treatment with l-asparagine;lanes 16–20: T1 lines (line 11-1, line 12-3, line 13-2, line 14-5, line 15-1)versus 11–15 T0 lines from treatment with l-glutamine;lanes 21–30: T1 lines(line 16-1, line 17-1, line 17-2, line 17-9, line 17-10, line 17-11, line 17-12, line 17-14, line 18-5, line 19-10) versus 16–19 T0 lines from treatment with both l-asparagine and l-glutamine).