Table 1.

Shoot induction frequenciesofthe different treatments.

Treatment	No. of Explants	No. of Explants with Shoots	Shoot Induction Frequency (%)
(−) no supplemental l-amino acid	390	270	68.9 ± 2.0 d
(+) l-asparagine	407	316	77.9 ± 2.0 c
(+) l-glutamine	358	304	84.3 ± 1.3 b
(+) l-asparagine and l-glutamine	431	400	92.5 ± 2.6 a

Three independent experiments were performed for each treatment. No. of explants: the total number of explants for the three experiments; No. of explants with shoots: the total number of explants produced shoots for the three experiments. The explants with shoots were defined as the shoots with green leaves after 21 days transferred to selection media. The shoot induction frequency is expressed as the mean ± standard deviation. The different small letters represent significant differences between treatments by ANOVA (p < 0.05). (–) represents no L-amino acid added into the media. (+) represents L-amino acid added into the media.