Table 3.
No. of elongated shoots at different times and elongation rates in the different treatments.
| Treatment | No. of Explants | No. of Elongated Plants (Percentage, %) | Elongation Rate % | |||||
|---|---|---|---|---|---|---|---|---|
| Total | SE1 | SE2 | SE3 | SE4 | SE5 | |||
| (−) no supplemental l-amino acid | 390 | 74 | 21 (28.4) | 28 (37.8) | 25 (33.8) | - | - | 20.2 ± 5.6 b |
| (+) l-asparagine | 407 | 162 | 56 (34.6) | 71 (43.8) | 31 (19.1) | 4 (2.5) | - | 39.8 ± 1.9 ab |
| (+) l-glutamine | 358 | 116 | 16 (13.8) | 43 (37.1) | 40 (34.5) | 13 (11.2) | 4 (3.4) | 26.6 ± 11.1 ab |
| (+) l-asparagine and l-glutamine | 431 | 180 | 24 (13.3) | 104 (57.7) | 35 (19.5) | 17 (9.5) | - | 41.6 ± 18.2 a |
Three independent experiments were performed for each treatment. No. of explants: the total number of explants for the three experiments; No. of elongated plants: the total number of elongated plants for the three experiments. SE1: the first time; SE2: the second time; SE3: the third time; SE4: the fourth time; SE5: the fifth time. Percentage % = No. of elongated shoots produced at a specific time/Total number ×100%. The elongation rate is expressed as the mean ± standard deviation. The different small letters represent significant differences between treatments by ANOVA (p < 0.05). (–) represents no l -amino acid added into the media. (+) represents L-amino acid added into the media.