Table 4.

Detection of elongated plants and transformation frequency for the different treatments.

Treatment	No. of Explants	No. of Elongated Plants	No. of Positive Plants	Positive Rate of Elongated Plants (%)	Transformation Efficiency (%)
(−) no supplemental l-amino acid	390	74	12	16.2 ± 6.8 b	3.5 ± 2.4 c
(+) l-asparagine	407	162	22	14.8 ± 4.8 b	5.9 ± 2.1 b,c
(+) l-glutamine	358	116	32	36.0 ± 11.6 a	8.8 ± 1.5 a,b
(+) l-asparagine and l-glutamine	431	180	46	30.2 ± 14.9 a,b	11.1 ± 2.7 a

Each treatment has three independent experiments. No. of infected explants: the total number of infected explants for the three experiments; No. of elongated explants: the total number of elongated explants for the three experiments. No. of positive plants: the total number of plants with PAT strips⁺. Positive rate of elongated plants % = No. of positive plants/No. of elongated explants × 100%. The transformation frequency % = the No. of positive plants/No. of infected explants × 100%. The Positive rate of elongated plants and transformation frequency are expressed as the mean ± standard deviation. The different small letters represent significant differences between treatments by ANOVA (p < 0.05). (–) represents no L-amino acid added into the media. (+) represents L-amino acid added into the media. ⁺ represents positive.