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Different stages of sorghum transformation: (a) immature Embyos after 14 days of pollination; (b) explant source-immature embryos; (c,d) explant source-immature inflorescence; (e,f) callus induction from immature inflorescence; (g) regeneration of shoo;t (h) regeneration of root; (i) excessive Agrobacterium growth around explant seize the callus induction; and (j) production of phenolic compounds resulted in ultimate death of callus.
