Figure 1.

Western Blot analysis of Ferritin Heavy Chain (FHC) and nuclear p65 expression in K562 and SKOV3 cell lines. (a) Western Blot analysis of FHC and nuclear p65 expression in K562^shScr, K562^shFHC and K562^shFHC/pcFHC cells. (b) Western Blot analysis of phospho-IκBα S32/36 in K562^shScr and K562s^hFHC cells. (c) Western Blot analysis for FHC and nuclear p65 expression upon Ferlixit^® treatment of K562^shScr and K562^shFHC cells. (d) Western Blot analysis of FHC and nuclear p65 expression in SKOV3^shScr, SKOV3^shFHC and SKOV3^shFHC/pcFHC cells. For all panels, FHC expression analysis was performed on 40 µg of total protein, while nuclear p65 expression was performed on 40 µg of nuclear protein extracts. γ-Tubulin, IκBα and HDAC were used as loading control for total or nuclear protein levels, respectively. Blots are representative of three independent experiments. The graph represents the mean of the optical densities (* p < 0.05).