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Elution profile of fraction F from Figure 1 by reversed phase-high performance liquid chromatography (RP-HPLC). Column: Synergi 4u Hydro-RP 80A (10 × 250 mm2; particle size: 4 μm; Phenomenex); elution A (deionised water containing 0.1% trifluoroacetic acid) and B (100% acetonitrile containing 0.1% trifluoroacetic acid); mobile phase: a linear gradient from 0% to 40% of B within 120 min; flow rate of 1.5 mL/min at room temperature, and detection at 220 nm.
