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. 2018 Oct 9;19(10):3078. doi: 10.3390/ijms19103078

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Three-mon post-¹²C irradiation, there was no difference in the number of Ki67+ cells or clusters between IRR and Sham mice. (A) Representative photomicrograph of Ki67 staining in a Long-Term Sham mouse (mouse #13850; 400×, scale bar = 25 µm). (B) An unpaired t-test revealed no difference in the number of Ki67+ cells in the SGZ (p > 0.05; N = 4 Sham, N = 8 IRR). (C) An unpaired t-test revealed no difference in the number of Ki67+ clusters in the SGZ (p > 0.05). (D) Bregma analysis of Ki67+ cells in the SGZ through two-way ANOVA (Bregma × Treatment) revealed a main effect of Bregma (F_11,110 = 5.509; **** p < 0.0001), but not of Treatment (F_1,10 = 0.2151; p > 0.05) and no significant interaction (F_11,110 = 0.4856; p > 0.05). Post-hoc (Sidak’s multiple comparison) test revealed no significance at any single bregma. Error bars ± SEM.