Table 2.

A comparison of 24 residues in the hydrophobic cavity of TbAOX, hexaploid wheat AOX (TaAOX), Triticum urartu AOX (TuAOX, wild diploid wheat ancestor), Aegilops tauschii AOX (AetAOX, wild diploid wheat ancestor), HvAOX, and OsAOX. The nine residues common amongst all the protein isoforms are excluded. The wheat residues are from Brew-Appiah et al. [12], and the barley and rice residues are from Wanniarachchi et al. [14]. dG1 and dG2 refer to AOX1d Group 1 and AOX1d Group 2 respectively. * Indicates diploid wheat isoforms.

	AOX Isoforms	TbAOX Residues and Positions in the Hydrophobic Cavity
		S	T	C	W	L	F	S	R	F	P	L	V	S	I	T	I	M	F	L	A	I	F	V	F
		91	94	95	97	98	99	117	118	121	178	179	181	182	185	186	189	190	193	194	197	200	204	205	208
	HvAOX1a	T	S	L	F	P	T	C	R	M	Y	E	A	L	T	V	V	F	A	Y	G	I	F	A	V
	TaAOX1a-2AL	T	S	L	F	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	G	I	F	A	V
	TaAOX1a-2BL	T	S	L	F	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	G	I	F	A	V
	TaAOX1a-2DL	T	S	L	F	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	G	I	F	A	V
	TaAOX1a-like-2DL	-	-	-	-	-	-	-	-	-	Y	E	A	L	A	V	V	F	A	Y	G	V	F	A	V
	TuAOX1a *	T	S	L	F	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	G	I	F	A	V
	AetAOX1a *	T	S	L	F	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	G	I	F	A	V
	OsAOX1a	T	S	L	F	P	T	C	R	M	Y	E	A	L	T	V	V	F	A	Y	G	L	F	A	V
	HvAOX1c	T	S	L	V	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	G	L	F	A	V
	TaAOX1c-6AL	T	S	L	V	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	G	I	F	A	V
	TaAOX1c-6BL	T	S	L	V	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	G	V	F	A	V
	TaAOX1c-6DL	T	S	L	V	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	G	V	F	A	V
	TuAOX1c *	T	S	L	V	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	G	V	F	A	V
	OsAOX1c	T	A	L	V	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	G	L	L	A	V
	Put.TaAOX1e-3DS	T	A	I	W	P	T	C	R	M	Y	E	A	L	V	V	V	F	A	Y	T	A	V	A	M
	AetAOX1e *	T	A	M	W	P	T	C	R	M	Y	E	A	L	A	V	V	F	A	Y	T	A	V	A	M
	OsAOX1e	T	S	L	W	P	V	C	R	M	Y	E	A	L	A	V	A	F	A	Y	S	L	F	A	I
	OsAOX1d	T	S	L	V	P	R	S	H	L	W	E	A	L	A	A	V	F	A	Y	G	V	F	A	F
dG1	HvAOX1d1	I	T	L	A	G	S	S	H	L	W	E	A	L	A	T	V	F	A	Y	G	V	F	A	F
	TaAOX1d-2AL.1	I	T	L	A	G	S	S	H	L	W	E	A	L	A	T	V	F	A	Y	G	V	F	A	F
	put.TaAOX1d-like-4AS	-	-	-	-	-	-	S	H	L	C	E	A	L	P	T	V	F	A	Y	G	V	F	A	F
	TuAOX1d.2 *	I	T	L	K	G	S	S	H	L	W	E	A	L	A	T	V	F	A	Y	G	V	F	A	F
	AetAOX1d-like *	I	T	L	A	G	S	S	H	L	-	-	-	-	-	-	V	F	A	Y	G	I	L	-	-
dG2	HvAOX1d2	V	S	L	V	P	R	S	H	L	W	E	A	L	A	A	V	F	A	Y	G	I	F	A	F
	TaAOX1d-2AL.2	V	S	L	V	P	R	S	H	L	W	E	A	L	A	A	V	F	A	Y	G	I	F	A	F
	TaAOX1d-2DL	V	S	L	V	P	R	S	H	L	W	E	A	L	A	A	V	F	A	Y	G	I	F	A	F
	TuAOX1d.1 *	V	S	L	V	P	R	S	H	L	W	E	A	L	A	A	V	F	A	Y	G	I	F	A	F
	AetAOX1d *	V	S	L	V	P	R	S	H	L	W	E	A	L	A	A	V	F	A	Y	G	I	F	A	F