Table 3.

A comparison of residues in the dimerization interface of the TbAOX, hexaploid wheat AOX (TaAOX), Triticum urartu AOX (TuAOX, wild diploid wheat ancestor), Aegilops tauschii AOX (AetAOX, wild diploid wheat ancestor), HvAOX, and OsAOX. The wheat residues are from Brew-Appiah et al. [12], and the barley and rice residues are from Wanniarachchi et al. [14]. dG1 and dG2 refer to AOX1d Group 1 and AOX1d Group 2, respectively. * Indicates diploid wheat isoforms.

	AOX Isoforms	Completely Conserved with TbAOX						Semi-Conserved with TbAOX
		H138	L142	R143	R163	L166	Q187	M131	M135	L139	S141	M145	R147	D148	L156	A159	M167	R180	I183
	HvAOX1a	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	TaAOX1a-2AL	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	TaAOX1a-2BL	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	TaAOX1a-2DL	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	TaAOX1a-like-2DL	H	L	R	R	L	Q	M	V	L	S	F	H	S	M	A	M	R	V
	TuAOX1a *	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	AetAOX1a *	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	OsAOX1a	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	HvAOX1c	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	TaAOX1c-6AL	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	TaAOX1c-6BL	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	TaAOX1c-6DL	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	TuAOX1c *	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
	OsAOX1c	H	L	R	R	L	Q	M	M	L	S	F	H	S	L	A	M	R	V
	put.TaAOX1e-3DS	H	L	R	R	L	Q	M	A	L	S	F	Q	S	L	A	M	R	V
	AetAOX1e *	H	L	R	R	L	Q	M	A	L	S	F	Q	S	L	A	M	R	V
	OsAOX1e	H	L	R	R	L	Q	M	A	L	S	F	H	S	L	A	M	R	V
	OsAOX1d	H	L	R	R	L	Q	M	M	L	S	F	Q	S	L	A	M	R	V
dG1	HvAOX1d1	H	L	R	R	L	Q	M	V	L	S	F	H	S	M	A	M	R	V
	TaAOX1d-2AL.1	H	L	R	R	L	Q	M	V	L	S	F	H	S	M	A	M	R	V
	put.TaAOX1d-like-4AS	H	L	R	R	L	Q	M	V	L	S	F	H	N	M	A	M	R	V
	TuAOX1d.2 *	H	L	R	R	L	Q	M	V	L	S	F	H	S	M	A	M	R	V
	AetAOX1d-like *	H	L	R	R	L	-	M	V	L	S	F	H	S	M	A	M	-	-
dG2	HvAOX1d2	H	L	R	R	L	Q	M	V	L	S	F	H	S	M	A	M	R	V
	TaAOX1d-2AL.2	H	L	R	R	L	Q	M	V	L	S	F	H	S	M	A	M	R	V
	TaAOX1d-2DL	H	L	R	R	L	Q	M	V	L	S	F	H	S	M	A	M	R	V
	TuAOX1d.1 *	H	L	R	R	L	Q	M	V	L	S	F	H	S	M	A	M	R	V
	AetAOX1d *	H	L	R	R	L	Q	M	V	L	S	F	H	S	M	A	M	R	V