(A)

Inclusion Criteria	Exclusion Criteria
1.Metabolic and physical parameters: FBG ≤ 110 mg/dL; HbA1c ≤ 6.0%; TC ≤ 260 mg/dL; BMI ≤ 26 Kg/m2; age of ≥30 and ≤60 years. All parameters stable during the past 12 months.	1.Metabolic parameters: metabolic parameters as in the inclusion criteria met by medication; variation of one or more parameter by ≥10% (5% for HbA1c) of the value in the past 12 months.
2.Lifestyle: habitual Mediterranean diet *; sedentary to recreationally moderately active (≤2 days/week); ≤1 drink/day for women and ≤2 drinks/day for men **; non- or light-smoker (≤6 CPD).	2.Lifestyle: past or current self-imposed or prescribed special dietary regimes; history of eating disorders; daily or weekly physical routines of vigorous intensity.
3.Medical examination: in general, good health at the initial medical screening; BP ≤130/80 without anti-hypertensive medication; participation approved by the Medical Director of the study.	3.Medical history: past or current metabolic or endocrine disorders, cardiovascular, neurological or hematological diseases, chronic medication, history of special dietary regimes; family history of T2D and/or hyperlipidemia; planned or current pregnancy; planned surgical procedures during or immediately after the end of the study.

* As defined in refs [10,11,12]; ** Drink equivalents as defined in the 2015–2020 U.S. Dietary Guidelines for Americans; FBG = fasting blood glucose, HbA1c = glycated hemoglobin, TC = total cholesterol, BMI = body mass index, CPD= cigarettes per day, BP = blood pressure, T2D = type 2 diabetes mellitus.