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. 2018 Sep 28;19(10):2959. doi: 10.3390/ijms19102959

Figure 4.

Figure 4

Expression of slow and fast muscle-associated genes in quadriceps muscles. (A) Myosin heavy chain gene expressions by qRT-PCR. Myh7 is represented as a slow muscle type, whereas Myh2, Myh1, and Myh4 are fast muscle types. (B) Comparisons of protein levels associated with slow muscle types between WT and TG in quadriceps muscle by western blotting. Overall, slow muscle type associated proteins were up-regulated in quadriceps muscles of TG mice. (C) Analysis of slow muscle-associated genes expression by qRT-PCR. (D) Analysis of fast muscle-associated genes expression by qRT-PCR. These data were from four-month-old wild-type (n = 4) and transgenic mice (n = 5), which were normalized according to the amount of mouse Gapdh mRNA and expressed relative to the corresponding value of the wild-type mice. Data are expressed as the means ± SEM for three independent replicates and multiple t-tests were used for the statistical analysis. * = p < 0.05; ** = p < 0.01; *** = p < 0.01.