Table 2.

Amino acid differences between ZIKV-MY and ZIKV-Natal.

Protein	Amino Acid Position (Within Protein)	African, MR766 1947 (146× SM, 1× C6/36, 1× Vero)	African, Nigeria 1968 (21× SM, 1× Vero)	Malaysian ZIKV-MY 1966 (6× SM, 1× BHK, 1× C6/36, 8× Vero)	African, DakAr41525 1985 (5× Vero, 1× C6/36, 2× Vero)	Brazilian ZIKV-Natal 2015 (No Passaging)	Brazilian, Suriname 2015 (4× Vero)
prM	1	A	A	V	A	A	A
	17	S	S	S	S	N	N
	21	K	K	K	K	E	E
	31	V	V	V	V	M	M
E	154–156	NDT	Deleted	NDI	NDT	NDT	NDT
	393	D	D	D	D	E	E
	473	V	V	V	V	M	M
	487	T	T	T	T	M	M
NS1	146	E	K	K	K	E	K
	188	V	V	A	V	V	V
	233	T	T	T	T	A	T
	264	V	V	V	V	M	M
	349	M	M	M	M	V	M
NS2A	117	A	A	V	A	A	A
	143	A	A	A	A	V	V
NS3	400	N	N	N	N	H	H
	472	M	M	M	M	L	L
	584	Y	Y	Y	Y	H	H
NS4B	14	A	A	G	A	S	S
	26	M	V	M	M	I	I
	49	L	L	L	L	F	F
	98	M	M	M	M	I	I
	180	I	A	I	V	V	V
	184	V	V	V	V	I	I
	186	L	L	L	L	S	S
	240	T	T	T	T	I	T
NS5	115	M	M	T	M	V	V
	140	P	P	S	P	P	P
	230	I	I	I	I	T	T
	268	A	A	A	A	V	V
	276	M	M	L	M	M	M
	283	I	I	V	I	I	I
	377	N	N	N	N	S	S
	527	A	A	T	A	I	I
	531	K	K	K	K	R	R
	588	G	G	R	G	K	K
	643	P	P	P	P	S	S
	648	R	R	S	R	N	N
	704	S	S	S	S	D	D
	714	H	H	Y	H	H	H
	720	Y	H	Y	H	H	H
	868	D	D	D	D	N	N

Accession numbers: ZIKV-MY (KX694533), ZIKV-Natal (KU527068), MR766 (HQ234498), Nigeria (HQ234500) DakAr41525 (KU955591), Suriname (KU312312). SM—suckling mouse brain.