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. 2018 Oct 26;12:92. doi: 10.3389/fncir.2018.00092

FIGURE 2.

FIGURE 2

Cells labeled with fluorescent proteins after electroporation and Adeno-associated virus (AAV) injection. (A) Yellow fluorescent protein (YFP)-labeled tadpole neurons 1 day after plasmids were electroporated in vivo. (B) Transverse section of left telencephalon of adult X. laevis 5 days after plasmids were electroporated in vivo. LV indicates lateral ventricle. (C) Green fluorescent protein (GFP)-expressing cells (inset shown in C) that lack processes of neurons, presumed to be glial cells. (D) Labeled processes of glial-like cells (inset shown in C) that seem to form the ependymal lining of the ventricle. (E) Vimentin-positive radial glial cells in the adult telencephalon. (F) YFP-labeled telencephalic neurons of one of the very few brains that expressed reporter genes in response to AAV injection. (G) Membrane potential of a presumed glial cell electroporated with plasmid (pCS2FA.ChR2.YFP) positive for YFP and Channelrhodopsin 2 (ChR2). In response to blue light exposure (indicated in blue lines below the trace), the membrane potential depolarized, presumably because of the ChR2 expression, but the cell never spiked an action potential.