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. 2018 Oct 26;12:92. doi: 10.3389/fncir.2018.00092

FIGURE 5.

FIGURE 5

Neurons transduced by vesicular stomatitis virus (VSV) around the injection site. (A) Neurons with reporter gene expression near the injection site of recombinant vesicular stomatitis virus encoding its own glycoprotein [rVSV(VSV-G)] in the telencephalon 2 days post infection. There was a robust expression of reporter genes about 400 μm surrounding the injection site. A white arrow indicates the injection site. (B) In addition to labeled somata, there are dense processes that are labeled around the injection site. (C) The nerve injection site shown in the isolated brain. (D) Cross section of the laryngeal nerve 24 days post injection. (D1) Enlarged section of perforated rectangle shown in (E). Note that the labeling is concentrated on the periphery of the axons (a, in inset on the right). (E) The horizontal section of the brainstem of a male X. laevis with rVSV(VSV-G) injected into the cranial nerve IX-X 24 days prior. The white line encircle the n.IX-X. There was no labeled neurons nor axons found in the brainstem or the nerve. (E1) Enlarge section of a white perforated rectangle shown in (E). Labeled cells seen are autofluorescent red blood cells in the blood vessels (amphibian red blood cells are nucleated).