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. 2018 Oct 26;12:92. doi: 10.3389/fncir.2018.00092

FIGURE 8.

FIGURE 8

A test for the transsynaptic spread of recombinant vesicular stomatitis virus encoding rabies glycoprotein [rVSV(RABV-G)]. (A) A dorsal view of the X. laevis forebrain illustrating the connection among laryngeal motor nucleus (n.IX-X), dorsal tegmental area of medulla (DTAM) and the bed nucleus of the stria terminalis (BNST); n.IX-X receives projections from ipsilateral DTAM, which in turn, receives input from the ipsilateral bed nucleus of the BNST. (B) A horizontal section of the anterior brainstem showing labeled neurons in ipsilateral DTAM 10 days post infection. White dotted line encircle DTAM. (C) Horizontal section of the caudal telencephalon showing the location of BNST. (D) An enlarged section in C showing the absence of labeling in the ipsilateral BNST 10 days post injection.