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. 2018 Sep 25;19(10):2900. doi: 10.3390/ijms19102900

Figure 1.

Figure 1

Construction and transformation of the pPICZ-αA/BsDb expression plasmid. (A) Schematic diagram of the pPICZ-αA/BsDb expression plasmid generation. The gene fragment of BsDb containing a 6His-tag at the C-terminal end was cloned into pPICZαA by using EcoRI/XbaI digestion; (B) Restriction enzyme digestion of recombinant pPICZαA-BsDb expression vector. Lane M, 1 k bp marker (Thermo, Waltham, MA, USA); (C) Colony PCR analysis of 20 positive recombinants obtained from YPD plates containing zeocin. Lane M, 1 k bp marker (Thermo, Waltham, MA, USA); (D) Colony PCR analysis of a negative clone transformed pPICZαA empty vector. Lane M, 100 bp marker (Thermo, Waltham, MA, USA).