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. 2018 Sep 25;19(10):2900. doi: 10.3390/ijms19102900

Figure 2.

Figure 2

Analysis of recombinant BsDb expression in Pichia pastoris. (A) SDS-PAGE analysis: culture supernatant from a negative clone induced by 1% methanol for 96 h (lane 1); culture supernatant from the positive transformant grown under identical condition (lane 2); culture supernatant concentrated 5-fold from the positive transformant (lane 3); and protein molecular weight marker (Sangon, Shanghai, China) (Lane M); (B) Western blot analysis: supernatant from a negative clone (lane 1); culture supernatant from a positive transformant (lane 2); and culture supernatant concentrated 5-fold from a positive transformant (lane 3).