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Purification of recombinant BsDb using Ni-NTA affinity chromatography. (A) SDS-PAGE of purified recombinant BsDb; (B) Western blot analysis of purified recombinant BsDb (lane 1) and 5 µg BSA was used as a negative control for Western blot analysis (lane 2), protein molecular weight marker (Sangon, Shanghai, China) (Lane M); and (C) HPLC analysis of purified recombinant BsDb.
