Figure 10.

BsDb suppressed tumor growth in vivo. (A) The tumor volume of xenograft mice during the treatment. BsDb exerted robust antitumor activity in vivo with a dose-dependent manner; (B,C) mice were sacrificed after treatment for 21 days and tumors were collected and weighed; (D) Serum levels of INF-γ in different groups of mice were detected using ELISA after treatment for 21 days. Data were expressed as the mean ± SD (n = 5); (E) Immunofluorescence staining of CD31 (red fluorescence) and INF-γ (green fluorescence) in sections from tumors of different groups of mice. The cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) in blue and the sections were photographed using a fluorescence microscope (200×); (F,G) quantitative analysis of CD31 and INF-γ fluorescence staining using ImageJ software. Data were expressed as the mean ± SD (n = 3). Statistical analysis was tested using one-way analysis variance (ANOVA) and post hoc Tukey honestly significant difference (HSD). * p < 0.05, ** p < 0.01, and *** p < 0.001 versus the control group; # p < 0.05 and ## p < 0.01 versus the 5 mg/kg group.