Figure 2.

Combined effect of MEL and ATRA on the viability and proliferation status of HL-60 cells. Cells were seeded in a 96-well plate at a density of 5 × 10³ cells per well and treated with 1 µM ATRA (column 2), and 10 nM ATRA (columns 3) and 1 mM MEL (column 4), and MEL in combination with 10 nM ATRA (column 5); untreated cells (control, column 1). (a) Cell viability in % relative to the control; (b) The mitotic index (MI) is defined as the ratio between the number of cells in mitosis and the total number of cells. The data are presented as the means ± S.D. of ten separate experiments. * p < 0.05 significant difference in values in comparison with the corresponding control, ^# p < 0.05 significant difference in values compared to the value obtained after the addition of MEL alone.