Figure 3.
Combined effect of MEL and ATRA on the level of Bcl-2 (a) and Bcl-xL (b) proteins in HL-60 cells. Cells were seeded in a 96-well plate at a density of 5 × 103 cells per well and treated with 1 µM ATRA (column 2), and 10 nM ATRA (columns 3) and 1 mM MEL (column 4), and MEL in combination with 10 nM ATRA (column 5); untreated cells (control, column 1). Protein samples were extracted and subjected to Western blot for Bcl-2 and Bcl-xL detection. Immunodetection of α-tubulin was used as a loading control. Upper parts-immunostaining of Bcl-2, Bcl-xL and α-tubulin. Lower part-quantitation of immunostaining using computer-assisted densitometry. Bar graphs represent the proteins levels in relative units. The protein level in a cell lysate without any addition was taken to be unity and served as a control. The data are presented as the means ± S.D. of five separate experiments. * p < 0.05 significant difference in the protein level compared with the corresponding control, # p < 0.05 significant difference in the protein level compared to the value that was obtained in the presence of MEL alone.