Figure 5.

Combined effect of MEL and ATRA on the level of CNPase in HL-60 cells. Cells were seeded in a 96-well plate at a density of 5 × 10³ cells per well and treated with 1 µM ATRA (column 2), and 10 nM ATRA (columns 3) and 1 mM MEL (column 4), and MEL in combination with 10 nM ATRA (column 5); untreated cells (control, column 1). Protein samples were extracted and subjected to Western blot for CNPase detection. The immunodetection of α-tubulin was used as a loading control. Upper part-immunostaining of CNPase and α-tubulin. Lower part-quantitation of immunostaining using computer-assisted densitometry. Bar graphs represent the Bcl-2 level in relative units. The protein level in a cell lysate without any addition was taken to be unity and served as a control. The data are presented as means ± S.D. of five separate experiments. * p < 0.05 significant difference in protein level in comparison with corresponding control, ^# p < 0.05 significant difference in the protein level compared to the value obtained in the presence of MEL alone.