Figure 6.

The effect of MEL and ATRA on the mitochondrial respiratory chain complexes in HL-60 cells. Cells were seeded in a 96-well plate at a density of 5 × 10³ cells per well and treated with 1 µM ATRA (column 2), and 10 nM ATRA (columns 3) and 1 mM MEL (column 4), and MEL in combination with 10 nM ATRA (column 5); untreated cells (control, column 1). Protein samples were extracted and subjected to Western blotting. Changes in mitochondrial complexes were detected using, the Total OXPHOS Rodent WB Antibody Cocktail. The immunodetection of α-tubulin was used as a loading control. (a) Immunostaining with OXPHOS antibody cocktail and α-tubulin; (b–f)-quantification of immunostaining using computer-assisted densitometry. Bar graphs represent the levels of appropriate complexes (I–V) in relative units. The protein level in a cell lysate without any addition was taken to be unity and served as a control. The data are presented as means ± S.D. of five separate experiments. * p < 0.05 significant difference in protein level in comparison with the corresponding control, ^# p < 0.05 significant difference in the protein level compared to the value obtained in the presence of MEL alone.