Table 5.
Examples of expression vectors in C. glutamicum.
| Vector | Size (kb) | Replicon | Copy number per cell | Selection marker | Promoter, regulatory gene | Induction conditions (Conc.) | References |
|---|---|---|---|---|---|---|---|
| pEKEx1 | 8.2 | pBL1 | 10–30 | Kmr | Ptac, lacIq | IPTG (0.2 mM) | Eikmanns et al., 1991 |
| pXMJ19 | 6.6 | pBL1 | 10–30 | Cmr | Ptac, lacIq | IPTG (1 mM) | Anglana and Bacchetti, 1999 |
| pBKGEXm2 | 7.3 | pBL1 | 10–30 | Kmr | Ptac, lacIq | IPTG (1 mM) | Srivastava and Deb, 2002 |
| pCRA1 | 5.3 | pBL1 | 10–30 | Cmr | Plac | Constitutive | Nakata et al., 2003 |
| pCRA429 | 4.3 | pBL1 | 10–30 | Cmr | Ptac | Constitutive | Suzuki et al., 2009 |
| pDXW-8 | 9.6 | pBL1 | 10–30 | Kmr | Ptac, lacIPF104 | IPTG (1 mM) | Xu et al., 2010 |
| pEC901 | 8.5 | pCG1 | 30 | Kmr | PL/PR (λ), cI857 | 40°C | Makoto Tsuchiya, 1988 |
| pZ8-1 | 7.0 | pCG1 | 30 | Kmr | Ptac | Constitutive | Dusch et al., 1999 |
| pVWEx1 | 8.5 | pCG1 | 30 | Kmr | Ptac, lacIq | IPTG (1 mM) | Peters-Wendisch et al., 2001 |
| pSL360 | 6.5 | pCG1 | 30 | Kmr | P180 | Constitutive | Park et al., 2004 |
| pECXK99E | 7.0 | pGA1 | 30 | Kmr | Ptac, lacIq | IPTG (0.5 mM) | Kirchner and Tauch, 2003 |
| pTRCmob | 6.4 | pGA1 | 30 | Kmr | Ptrc | IPTG (0.2 g/Lmedium) | Liu et al., 2007 |
| pAPE12 | 4.6 | pNG2 | <10 | Kmr | Ptac, lacIq | IPTG (0.15 g/Lmedium) | Guillouet et al., 1999 |