Table 1. Macromolecule-production information.
Source organism | P. halocryophilus |
Expression vector | pET-30 Ek/LIC |
Expression host | E. coli BL21 (DE3) |
Complete amino-acid sequence of the construct† | MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKMNTAIKVIHTLKAAGFEAYIVGGAVRDLLLGKTPHDVDVASSALPQQVKVLFDRTVDTGIDHGTVLVLLDGEGIEVTTFRTESSYSDNRRPDSVEFVLSLEEDLRRRDFTINAMAMTEDLKIIDPFGGKEDLKNKVIRAVGDPDERFEEDALRMLRAIRFSGQLDFIIDMKTLLSIRRHARLIRFIAVERLKSEIDKIFVNPSMQKSMAYLKDSVLTRFLPVGGLFEVDWITYHTDGNPTYGWLYLLHQQKRQFTDIKDYRFSNEEKRLIEKSLELTALNTWDQWTFYKYTLKQLEMASRVTGKKKDLAAIKRQLPIQSRSELAVDGWDLIEWSGAKSGPWLKVWIEKIERLIVYGILKNDKELIKDWFEDEYHSHT |
No. of amino acids | 420 |
Molecular mass (Da) | 48423 |
Abs 0.1%‡ at 280 nm (M −1 cm−1) | 1.36 |
The purification tag, including 6×His, is underlined.
Equal to a 1 g l−1 solution.