. 2018 Oct 31;74(Pt 11):747–753. doi: 10.1107/S2053230X18014590

Table 2. Crystallization conditions for PhaCCA.

Method	Counter-diffusion	Vapor diffusion
Container	Glass capillary in GCB-Domino	CrystalQuick plate
Temperature (K)	293	293
Protein concentration (mg ml⁻¹)	4.5	5
Composition of protein buffer solution	50 mM Tris–HCl pH 7.5, 200 mM NaCl, 5 mM MgCl₂	50 mM Tris–HCl pH 7.5, 200 mM NaCl, 5 mM MgCl₂
Composition of crystallant solution	3 M ammonium sulfate, 100 mM sodium acetate pH 5	A8: 20%(m/v) PEG 3350, 200 mM lithium sulfate pH 6.6
		B12: 20%(m/v) PEG 3350, 200 mM potassium citrate pH 8.3
		E8: 100 mM sodium acetate, 1 M diammonium hydrogen phosphate pH 4.5
Volume of sample	1.5 µl	200 nl
Volume of reservoir	1 ml	50 µl