Table 1. F. nucleatum NanA production information.

Source organism	F. nucleatum strain ATCC 25586
DNA source	Synthetic DNA
Forward primer	CAAAAAAGCAGGCTTCATGAAAGGGATATATTCAG
Reverse primer	CAAGAAAGCTGGGTTTTAATTTTTTAAAAATTTTTTATG
Cloning vector	pMK vector
Expression vector	pET300/NT-DEST with an N-terminal His tag
Expression host	E. coli BL21 (DE3)
Sequence of the recombinant protein produced†	MHHHHHHITSLYKKAGFMKGIYSALMVPYNEDGSINEKGLREIIRYNIDKMKVDGLYVGGSTGENFMISTEEKKRVFEIAIDEAKDSVNLIAQVGSINLNEAVELGKYVTKLGYKCLSAVTPFYYKFDFSEIKDYYETIVRETGNYMIIYSIPFLTGVNMSLSQFGELFENEKIIGVKFTAGDFYLLERVRKAFPDKLIFAGFDEMLLPATVLGVDGAIGSTYNINGIRAKQIFELAKNSKIDEALKIQHTTNDLIEGILSNGLYQTIKEILKLEGVDAGYCRKPMKKISQKQIEFAKELHKKFLKN

^†Non-native amino-acid residues originating from the vector are underlined.