Table 2. Crystallization of F. nucleatum NanA.

	Ligand-free FnNanA	Ligand-bound FnNanA with a pyruvate Schiff base
Method	Vapour diffusion, hanging drop	Vapour diffusion, hanging drop
Plate type	96-well	96-well
Temperature (K)	277	277
Protein concentration (mg ml−1)	10	10
Buffer composition of protein solution	50 mM HEPES pH 6.8, 50 mM NaCl, 10 mM β-mercaptoethanol	50 mM HEPES pH 6.8, 50 mM NaCl, 10 mM β-mercaptoethanol
Buffer composition of reservoir solution	0.1 M CHES pH 9.5, 10%(w/v) PEG 3000, 5 mM adenosine 5′-triphosphate disodium salt hydrate (ATP)	0.1 M CHES pH 9.5, 10%(w/v) PEG 3000, 2.85 mM sodium pyruvate
Volume of drop (nl)	700	700
Volume of reservoir (µl)	100	100