Table 5. Kinetic parameters of FnNanA and previously characterized N-acetylneuraminate lyases from E. coli (EcNanA), P. multocida (PmNanA) and methicillin-resistant S. aureus (MRSA NanA).

Enzymes	K m (mM)	k cat (s−1)	k cat/K m (s−1 mM −1)
FnNanA†	6.0 ± 0.6	12.5 ± 0.5	2.1
EcNanA‡	2.5 ± 0.3	10 ± 0.4	4
PmNanA‡	4.9 ± 0.7	16 ± 1	3
MRSA NanA§	3.2 ± 0.1	22 ± 0.1	6.9

^†Current study.

^‡Data taken from Uchida et al. (1984 ▸).

^§Data taken from North et al. (2016 ▸).