Table 2. Kinetic parameters for HBD from C. acetobutylicum .

ND; not detectable for calculations.

	NADH			Acetoacetyl-CoA
CacHBD	K m (µM)	k cat (s−1)	k cat/K m (µM s−1)	K m (µM)	k cat (s−1)	k cat/K m (µM s−1)
Wild type	31.4 ± 4.9	8.5 × 103 ± 9.3 × 102	2.7 × 102 ± 1.2 × 10	22.7 ± 4.6	1.3 × 104 ± 1.1 × 103	6.3 × 102 ± 8.0 × 10
S117A	ND	ND	ND	ND	ND	ND
H138A	16.8 ± 1.2	93.8 ± 1.9	5.6 ± 0.3	9.6 ± 0.9	104.3 ± 2.2	11.0 ± 0.7
N188A	ND	ND	ND	ND	ND	ND