Table 1.
Quantitative summary of transcriptome along with annotations (A) sequencing and assembly; (B) functional annotation; (C) translation summary of the transcriptome; and, (D) completeness assessment of the transcriptome.
| A. Sequencing and Assembly | ||||
|---|---|---|---|---|
| Technology | PacBio (Iso-Seq) | Illumina (Hi-Seq) | ||
| Reads | Bases (MB) | Reads | Bases (GB) | |
| Raw Sequence (HQ * Reads) | 324,602 | 700 | 1,273,902,514 | 128.8 |
| Processed Sequence | 324,602 | 700 | 1,235,970,946 | 125.8 |
| Denovo Assembled Contigs (N50) | 120,926 (3117) | 302 | ||
| Reference Mapped | 79.7% (Average of mapped reads) | |||
| Expressed | 59,135 | |||
| B. Annotations | ||||
| # of Sequences | # of Reference IDs | |||
| BLAST Hits | 93,672 (77.5%) | 29,862 | ||
| Gene Ontology | 69,399 (57.4%) | 3587 | ||
| KEGG Enzymes | 13,187 (11%) | 675 | ||
| C. Transdecoder | ||||
| Total Transcripts | 103,043 (85.2%) | |||
| Complete | 83,384 (68.9%) | |||
| 5′ Partial | 15,913 (13.1%) | |||
| 3′ Partial | 3664 (3%) | |||
| Internal | 82 | |||
| D. BUSCO | ||||
| Total Core Genes | 1440 (100%) | |||
| Complete | 1185 (82.3%) | |||
| Fragmented Core Genes | 75 (5.2%) | |||
| Missing Core Genes | 180 (5.2%) | |||
* HQ: PacBio high-quality consensus reads.