Figure 12.

Diagram illustrating 4 different I/R experimental protocols. (a) Heart tissues subjected to 45 min ischemia and 120 min of reperfusion, after 2 weeks of oral animal treatment with Saline and different doses of Sitagliptin, for infarct size measurement. (b) Hearts subjected to 45 min ischemia and 10 min brief reperfusion, after which the animals received a 2 weeks’ oral administration of Saline and Sitg (50 mg), for further biochemical measurements. Infarct size measurement from heart tissues exposed to prolonged ischemia-reperfusion injury, after which the animals received a 2 weeks’ co-treatment of Saline, Sitg (50 mg) and intraperitoneal injection of NOS-inhibitor (l-NAME) (c). In the 4th experimental protocol (d), the inhibitory effect of TRPV-1 against ischemia-reperfusion (IR) injury was assessed by heart infarct size measurement at the end of a prolonged reperfusion-injury, and after co-treating the animals intraperitoneally with Capsazepine (TRPV-1 inhibitor).