Table 2.
Drought-responsive maize seedling leaf proteins observed specifically in tolerant line YE8112.
| No. | Protein ID 1 | Gene Name/ID 2 | Description 3 | Coverage (%) 4 | Peptide Fragments 5 | Fold Change 6 | p Value 7 | Pathways 8 |
|---|---|---|---|---|---|---|---|---|
| 1 | C0HJ06 | 541618 | Uncharacterized protein 9 | 22.4 | 1 | 1.37 | 0.0109 | MAPK signaling pathway/Plant hormone signaling |
| 2 | Q41746 | Lhcb5-1 | Chlorophyll a-b binding protein, chloroplastic | 55.8 | 10 | 1.24 | 0.0131 | X3 |
| 3 | C0HGH7 | 100193714 | Universal stress family protein | 20.4 | 3 | 1.23 | 0.0430 | |
| 4 | A0A1D6GAZ6 | ZEAMMB73_Zm00001d012677 | Glycerophosphodiester phosphodiesterase GDPD5 | 16.8 | 5 | 1.22 | 0.0136 | Glycerophospholipid metabolism |
| 5 | C0P948 | Zm00001d024886 | Uncharacterized protein | 55.9 | 20 | 1.21 | 0.0350 | |
| 6 | A0A1D6PQ00 | 100286059 | U2 snRNP auxiliary factor large subunit | 9.4 | 2 | 0.83 | 0.0171 | Spliceosome |
| 7 | A0A1D6IUI1 | 100383306 | Ubiquitin carboxyl-terminal hydrolase 13 | 2.7 | 3 | 0.83 | 0.0217 | |
| 8 | A0A1D6MJP2 | ZEAMMB73_Zm00001d039613 | Uncharacterized protein | 19.9 | 4 | 0.82 | 0.0111 | |
| 9 | B4FTP2 | ZEAMMB73_Zm00001d021334 | Thioredoxin-like protein CDSP32 chloroplastic | 23.7 | 6 | 0.81 | 0.0246 | |
| 10 | B4F845 | 100191245 | Uncharacterized protein | 3.0 | 1 | 0.81 | 0.0027 | |
| 11 | H9BG22 | 101027254 | Alpha-dioxygenase | 4.4 | 3 | 0.80 | 0.0162 | alpha-linolenic acid metabolism |
| 12 | Q5GJ59 | TPS7 | Terpene synthase 7 | 14.8 | 5 | 0.78 | 0.0179 | |
| 13 | C0PHF6 | 100383595 | AAA-ATPase ASD mitochondrial | 10.6 | 5 | 0.55 | 0.0487 |
1 Protein ID, unique protein identifying number in the UniProt database; 2 Gene name/ID; name or ID number of the corresponding gene of the identified differentially abundant protein as searched against the maize sequence database Gramene (http://ensemble.gramene.org/Zea mays); 3 Description, annotated biological functions based on Gene Ontology (GO) analysis; 4 Coverage (%), sequence coverage is calculated as the number of amino acids in the peptide fragments observed divided by the protein amino acid length; 5 Peptides fragments, refer to the number of matched peptide fragments generated by trypsin digestion; 6 Fold change, is expressed as the ratio of intensities of up-regulated or down-regulated proteins between drought stress treatments and control (well-watered conditions); All the fold change figures below 1 represents that the proteins were down-regulated. All the figures above 1 means the proteins were up-regulated; 7 p value, statistical level (using Student’s t-test) below <0.05, at which protein differential expression was accepted as significant; 8 Pathways, metabolic Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in which the identified protein was found to be significantly enriched; 9 uncharacterized protein, a protein without any functional annotations ascribed to it at the present.