Table 1.
Experimental design.
| In Vitro Studies of Myogenic Potential | |||
| Proliferation Assays | Sample | days | Medium |
| Cell Viability | PBCE, P82 and 73 (film, micro- and sub-micro) |
1–7 | PM |
| Immunofluorescence | 1 | ||
| SEM analysis | 7 | ||
| Differentiation Assays | Sample | days | Medium |
| Real-Time PCR | P73 (film, micro- and sub-micro) | 7–14 | DM |
| Immunofluorescence | 14 | ||
| Enzyme-linked immunosorbent assay (ELISA) | 14 | ||
| In Vivo Biocompatibility | |||
| In vivo Biocompatibility | Sample | weeks | Murine Model |
| Scaffold Implantation | P73 (micro) | 4/6 | Wild type C57BL/6 Nude and scid/mdx |
| Histology & Histochemistry | |||
| Tissue Immunostaining | |||
PBCE = poly(butylene1,4-cyclohexandicarboxylate; P82 = P80/20; P73 = P70/30; PM = Proliferation Medium; DM = Differentiation Medium.