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. 2018 Oct 18;19(10):3221. doi: 10.3390/ijms19103221

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Paclitaxel (Tax) exerted different killing effects on different NSCLC cell lines, which was related to the degree of autophagy induction. (A) Cell morphology after 24-h treatment with 0.1 μM or 1 μM Tax. (B,C) Cell Counting Kit-8 (CCK-8) determination of viability of Tax-treated NCI-H1299 (D) and NCI-H1650 (D) cells. The median inhibitory concentration (IC50) was estimated by log(inhibitor) vs. normalized response nonlinear fit (GraphPad Prism 6.0). (D) Western blot evaluation of LC3 and p62 proteins in cells treated with Tax for 24 h. *** p < 0.001.