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. 2018 Oct 12;19(10):3147. doi: 10.3390/ijms19103147

Figure 1.

Figure 1

Cresyl violet stained rat brain sections and statistical evaluation of changes in number of vital neurons in the CA1 (cornu ammonis 1) region of hippocampus and M1 (primary motor cortex) region of rat brain cortex. Bright-field micrographs of CA1 region of hippocampus representing naive control (A), homocysteine-treated control (Hcy-C) (B), naive ischemia followed by 72 h of reperfusion (IR-72h) (C), Hcy-IR-72h (D) and micrographs of M1 region of rat brain cortex representing naive control (E), Hcy-C (F), naive IR-72h (G) and Hcy-IR-72h (H). Arrows indicate morphologically changed neurons, while arrow heads show vital neurons. Bar = 50 µm, n = 5/group. Schematic coronal rat brain section (according to Paxinos and Watson [34]) showing CA1 area of rat hippocampus (red rectangle) and M1 region (blue rectangle) of cerebral cortex. Number of vital neuronal cells in the CA1 area of hippocampus (I) and M1 region of rat brain cortex (J) in naive control, Hcy-C, naive IR-72h and Hcy-IR-72h. Results are presented as mean ± SEM, n = 5/group. *** p < 0.001 versus the control value; ++ p < 0.01 and +++ p < 0.001 versus the control with Hcy; & p < 0.05 and &&& p < 0.001 versus the corresponding reperfusion period.