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. 2018 Oct 16;19(10):3191. doi: 10.3390/ijms19103191

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Examination of calcitonin gene-related peptide (CGRP)- (A) and Substance P (SubP)- (B) containing cells in WT and TSP-1^−/− mice by in vitro immunofluorescence microscopy analysis. The average percent area signaling for the protein of interest was measured using ImageJ (Fiji) due to the discrete nature of neurotransmitter-containing vesicles. The percent area of staining was also examined for β-tubulin staining (C) and compared across groups (D). n = 6 for all conditions. Scale bars represent 300 μm and * denotes a significant difference.