Figure 2.

Analysis of the metabolic parameters RER, locomotor activity, heat production and subcutaneous body temperature (from left to right, respectively) of the animals in the metabolic cages. While in the metabolic cages, animals remained on their assigned feeding regimen. (a) 24 h traces of the metabolic parameters, averaged per group. The timing of the meals for the 6M group are indicated along the x-axis. (b) Difference within metabolic parameters between light and dark phase for the ad libitum and 6M fed animals, i.e., asterisks and ns refer to light/dark differences within the ad libitum and 6M group. (c) Average 24 h values of the metabolic parameters for both groups of animals. Data are depicted as means ± SEM. χ = indicates that a significant interaction was found for Feeding × L/D, ns = non-significant, **** p < 0.0001, *** p < 0.001, * p < 0.05, n = 15–16 per group, except for subcutaneous body temperature (n = 3–5 per group). Locomotor activity is presented as arbitrary units (AU). ad lib = ad libitum fed animals (black lines and bars), 6M = animals fed according to the 6-meals-a-day feeding regimen (grey lines and bars). Shaded areas represent the dark phase.