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. 2018 Oct 21;19(10):3271. doi: 10.3390/ijms19103271

Figure 4.

Figure 4

ChIP analysis of ANAC074 binding to NRS1, NRS2, and MybSt1. PCR products amplified from the promoters of AT2G21320 (A), AT2G31880 (B), and AT3G13470 (C) were size fractionated by gel electrophoresis. The promoter of AT2G21320 contains NRS1 motifs and without NRS2, Mybst1 and NACR motifs; the promoter of AT2G31880 includes NRS2 and no NRS1 and MybSt1 and NACRS; and the promoter of AT3G13470 contains only MybSt1 and no NRS1, NRS2, and NACRS. Black lines represent the promoter regions for PCR amplification, and their positions in promoters are also shown. Input, Input DNA was used as the control; CHIP+: chromatin immunoprecipitated with anti-GFP antibody and PCR amplified; CHIP-: chromatin immunoprecipitated with HA antibody and PCR amplified.